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KMID : 0903520090520040397
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 4 p.397 ~ p.400
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Reduction of Shoot Hyperhydricity in Micropropagated Potato Plants via Antisense Inhibition of a chCu/ZnSOD Gene
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Lee Yong-Hwa
Kim Hyun-Soon
Kim Mi-Sun
Kim Yoon-Sik
Joung Hyouk
Jeon Jae-Heung
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Abstract
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The occurrence of hyperhydricity in transgenic potato plants, which carry a chloroplastic Cu/ZnSOD gene of the lily in either a sense or antisense orientation, was investigated during in vitro culturing. Hyperhydric symptoms included curled leaves with a succulent appearance that were mainly observed in Cu/ZnSOD sense plants. By contrast, antisense plants showed a low frequency of hyperhydricity and a high rate of ex vitro survival. Therefore, a knockdown approach for a chCu/ZnSOD gene could be a practical method to reduce hyperhydricity in plant micropropagation.
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KEYWORD
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ethylene, hyperhydricity, H2O2, micropropagation, potato, superoxide dismutase
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